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Action de l’acriflavine en ce qui concerne les levures
APPENDIX Within appendix news and you may buffers needed for the newest actions lead about before areas of which section is actually detailed. Mass media
Slonimski, P
BMM. step one.5 grams malt extract and you may 20 grams agar for the 1 L cornmeal pull. Cornmeal extract was extracted from 250 grams cornmeal incubated inside ten L h2o in the 60°C right-away. After this time new supernatant is actually filtered through several layers from cheesecloth, and also the cornmeal are discarded. CM typical. 0.15% MzP04,0.05% KCI, 0.05% MgS04,1% D- glucose, 0.37% NHEI, 0.2% Pepton, 0.2% fungus extract, step one yards& ZnS04,l mg/L FeCb Buffers Denaturation bufler: step one.5 Meters NaCI, 0.5 Yards NaOH Hybridization barrier: 50% Formamide (strict hybridization), 5 X SSPE, 0.5% salt dodecyl sulfate (SDS), 0.step 1 mg/mL salmon sperm DNA. (The fresh stringency regarding hybridization ide). (suite…)